Thermo Fisher Scientific CNOT4 Monoclonal Antibody (OTI3B8), TrueMAB
CNOT4 단백질을 인식하는 마우스 단클론 항체로, Western blot에 최적화됨. E. coli에서 생산된 인간 CNOT4 단백질(aa 190–455)을 면역원으로 사용. 1 mg/mL 농도의 액상 형태이며, PBS/BSA/glycerol 완충액에 보존. -20°C에서 보관, 동결-해동 반복은 피해야 함.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3B8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 190–455 of human CNOT4 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CNOT4 is a 575 amino acid protein with E3 ubiquitin ligase activity, mainly involved in protein modification and ubiquitination. It contains a C4C4-type RING zinc finger domain, an RNA recognition motif, and a bipartite nuclear localization signal. CNOT4 is a component of the CCR4-NOT (CAF1) core complex, which regulates transcription of multiple genes, influencing both activation and repression. It interacts with CNOT1 via its C-terminus and with E2 ubiquitin ligases via the RING domain. CNOT4 is phosphorylated in response to DNA damage at consensus sites recognized by ATM and ATR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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