Thermo Fisher Scientific HLA-DR/DP/DQ Monoclonal Antibody (CR3/43)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

CR3/43

Immunogen

Cells from human tonsil. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HLA-DR/DP/DQ,uniProtId:P01903-1,ncbiNodeId:9606,antigenRange:1-254,antigenLength:254,antigenImageFileName:A500-034A_HLA-DRDPDQ_P01903-1_House_mouse.svg,antigenImageFileNamePDP:A500-034A_HLA-DRDPDQ_P01903-1_House_mouse_PDP.jpeg,sortOrder:4}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

250 µg/mL

Purification

purified

Storage buffer

PBS, pH 6.8 to 7.4, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Immunocytochemistry: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Target Information

MHC class II molecules are transmembrane glycoproteins composed of an alpha chain (36 kDa) and a beta chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They therefore have a critical role in the initiation of the immune response.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.