Invitrogen™

One Shot™ PIR2 Chemically Competent E. coli

One Shot PIR2 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication자세히 알아보기

One Shot PIR2 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system). The pir gene encodes the replication protein π, which is required to replicate and maintain plasmids containing the R6Kγ origin. The PIR2 strain contains the wild-type pir gene that maintains the donor at ∼15 copies per cell. This strain is recommended for maintaining constructs that express toxic genes or for libraries. PIR2 is made chemically competent and can achieve high transformation efficiency of >1 x 108 cfu/μg supercoiled plasmid.

R6Kγ allows tight control and selection and has been used in various applications including genome engineering, mutagenesis studies, bacterial artificial chromosome (BAC) construction, high-capacity extrachromosomal vector systems, and suicide plasmid construction.

One Shot PIR2 Chemically Competent E. coli cells offer:
• Wild-type pir gene to maintain vectors that contain the R6Kγ ori at ∼15 copies per cell
• Transformation efficiencies of >1 x 108 cfu/μg
• end_A1 mutation that enables increased plasmid DNA yield and quantity
• _rec_A1 mutation that reduces the occurrence of unwanted recombination in cloned DNA
• _hsdR for efficient transformation of unmethylated DNA from PCR applications

Convenient and efficient format
PIR2 Chemically Competent E. coli cells are supplied in the convenient, single-reaction One Shot format. Each tube contains enough cells for one transformation, reducing freeze-thaw cycles and money wasted on unused cells.

Genotype
F–∆lac_169 _rpo_S(am) _rob_A1 _cre_C510 _hsd_R514 _end_A _rec_A1 _uidA(∆Mlu_I)::pir_
Genetic marker descriptions

Find the strain and format that fit your needs
Multiple DH strains are available in chemically competent and electrocompetent cell formats.
We offer the PIR1 strain that carries mutated pir gene and maintain vectors at ∼250 copies per cell.
MultiShot formats are available for high throughput applications.
Explore bacterial growth media formats.

사양

제품 유형Chemically Competent Cells

에프에피솜 포함No

플라스미드 품질 개선Yes (endA1)

메틸화 DNA 클로닝No

항생제 내성 박테리아No

블루/화이트 스크리닝No

고처리량 호환성Low

비메틸화 DNA 준비No

재조합 감소Yes (recA1)

배송 조건Dry Ice

T1 Phage - 저항성(tonA)No

종E. coli

형식Tube

제품라인One Shot™

수량11 x 50 μL/tube

Unit SizeEach