Thermo Fisher Scientific PLCG1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 700-800 of human PLC gamma 1 (PLCG1) (NP_002651.2).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.32 mg/mL

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914085

Product Specific Information

Positive test controls include: MCF7, Jurkat. The target is usually found in the following locations: Cell projection, lamellipodium, ruffle.

Immunogen sequence: NSYAISFRAE GKIKHCRVQQ EGQTVMLGNS EFDSLVDLIS YYEKHPLYRK MKLRYPINEE ALEKIGTAEP DYGALYEGRN PGFYVEANPM PTFKCAVKAL F

Target Information

The mammalian Phospholipase Cg (PLCg) family has two closely related proteins, PLCg1 and PLCg2. The PLCg isozymes have the core structure domains and a unique array of domains containing an additional PH domain, two SH2 domains and one SH3 domain. In response to extracellular stimuli, such as hormones and growth factors, receptor tyrosine kinases (RTKs) phosphorylate and activate PLCg. Activated PLCg catalyzes hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to produce the metabolic second messenger molecules inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.