Thermo Fisher Scientific ZMIZ1 Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Immunocytochemistry (ICC/IF)

• Tested Dilution: 0.25–2 µg/mL

Product Specifications

Product Specific Information

Immunogen sequence:
MNSMDRHIQQ TNDRLQCIKQ HLQNPANFHN AATELLDWCG DPRAFQRPFE QSLMGCLTVV SRVAAQQGFD LDLGYRLLAV CAANRDKFT

Highest antigen sequence identity to the following orthologs:

• Mouse: 100%
• Rat: 100%

Target Information

Retinoic acid plays a critical role in development, cellular growth, and differentiation and induces the expression of a variety of genes. RAI17 expression is induced by retinoic acid and is predominantly expressed in heart, brain, and ovaries. Within the brain, the highest expression is in the amygdala.

The deduced 1,067-amino acid protein contains an MSX-interacting zinc finger (MIZ) domain, a nuclear localization signal sequence, and two proline-rich regions. A strong intrinsic transactivation domain is identified in the C-terminal proline-rich region.

RAI17 expression is detected in various cancer cell lines and colocalizes with endogenous androgen receptor (AR) in the nuclei of prostate epithelial cells from human tissue samples. In human prostate cancer cells, RAI17 increases the transcriptional activity of AR. Studies using sumoylation-deficient AR mutants suggest that the increase of AR activity by RAI17 is dependent upon receptor sumoylation.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.