Invitrogen™

PureLink™ Total RNA Blood Kit

PureLink™ Total RNA Blood Kit allows purification of high-quality total RNA from 50 - 500 μl fresh, whole mammalian blood,자세히 알아보기

PureLink™ Total RNA Blood Kit allows purification of high-quality total RNA from 50 - 500 μl fresh, whole mammalian blood, using non-phenol based purification in a simple spin column based protocol.
• RNA purification from whole blood in under an hour
• Yields high-quality RNA devoid of non-nucleated erythrocytes

Simple RNA Purification from Whole Blood
PureLink™ Total RNA Blood Kit is designed for purifying from 50 to 500 μl fresh whole blood or approximately 3.5 x 106 leukocytes (white blood cells) in less than 1 hour. The entire protocol can be done in spin tubes and spin columns.

Leukocyte Enrichment for Improved RNA Purification
Whole blood consists of non-nucleated erythrocytes (red blood cells, 4 - 6 million/mm3) and leukocytes, with the latter present in the minority (5000 - 7000/mm3). Purifying total RNA from whole blood without the removal of erythrocytes results in low RNA yields and clogging of purification columns. The distinct feature of the PureLink™ Total RNA Blood Kit is that it employs a leukocyte enrichment step. Hypotonic shock is used to selectively lyse erythrocytes while not affecting leukocytes. Non-nucleated erythrocytes are efficiently depleted, thus significantly reducing cell debris that may otherwise interfere with RNA purification and other downstream applications. Consequently, yields of high-quality total RNA are obtained using the PureLink™ Total RNA Blood Kit.

Purified RNA is Suitable for Use with a Variety of Downstream Applications and Products:
• Northern Blotting Kits & Reagents
• Nuclease protection assays
• Microarray
• cDNA synthesis
• Real-Time PCR (qPCR)

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

사양

Isolation TechnologySilica Spin Column

정제 시간20 min. (After sample homogenization)

용리량30–100 μL

샘플 종류Blood

최종 제품 유형Total RNA

고처리량 호환성Not High-throughput Compatible (Manual)

용도(애플리케이션)RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling

수량50 preps

스케일Mini

배송 조건Room Temperature

시작 물질 양Blood: ≤500 μL

수율1000 μg (Binding capacity)

Unit SizeEach