Thermo Fisher Scientific GADD45B Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

Assay-Dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the middle region of human GADD45B

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2610079

Product Specific Information

Peptide sequence: FCCDNDINIV RVSGMQRLAQ LLGEPAETQG TTEARDLHCL LVTNPHTDAW

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100%

Target Information

Cellcycle progression issubject to arrest at G1 and G2 checkpointsin response to DNA damage, presumablyto allow time for DNA repair prior to entryinto S andMphase, respectively. The p53 tumorsuppressor isrequired for one such G1 checkpoint and functionsto upregulate expression of GADD 45 and p21. GADD 45 binds both Cdks and PCNA, a protein involved in DNA replication and repair. GADD 45 stimulates DNA excision repair in vitro and inhibits entry ofcellsinto S phase. Thus, it has been suggested that GADD 45 mayserve as a link between the p53-dependentcellcycle checkpoint and DNA repair. GADD 45-like proteins, GADD 45 beta and GADD 45 gamma, have been shown to be induced by environmentalstresses. GADD 45 beta and GADD 45 gamma are thought to induce p38/JNK activation via MEKK4 activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.