Thermo Fisher Scientific Cytokeratin 14 Polyclonal Antibody
Cytokeratin 14에 특이적인 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF 등 다양한 응용에 적합. 인간 및 랫트 반응성 확인됨. 고순도 항원 친화 크로마토그래피 정제. 장기 보관은 -20°C 권장.
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Thermo Fisher Scientific Cytokeratin 14 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | 1 publication |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 112–472 of Human Cytokeratin 14 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.48 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545478 |
Product Specific Information
- Recommended positive controls: HeLa, rat olfactory epithelium
- Predicted reactivity: Mouse (95%), Rat (94%), Dog (96%), Bovine (96%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (e.g., skin) and non-keratinizing cells (e.g., mesothelial cells). At least 20 different CKs can be identified.
Biochemically, CKs fall into two classes:
- Type I (acidic polypeptides)
- Type II (basic polypeptides)
Keratin 14, belonging to the type I subfamily, exists in combination with keratin 5 and distinguishes stratified epithelial cells from simple epithelial cells. It is useful in identifying squamous cell carcinomas and is considered a prognostic marker in breast carcinomas.
At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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