Thermo Fisher Scientific Acetyl-HIST1H2BC (Lys120) Polyclonal Antibody
Rabbit polyclonal antibody recognizing acetylated Histone H2B (Lys120) in human samples. Suitable for ICC/IF, ELISA, and ChIP assays. Purified by antigen affinity chromatography. Supplied as liquid form with PBS/glycerol buffer for research use only.
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- PA5144352
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- pk
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Immunocytochemistry (ICC/IF) | 1:1–1:10 | |
| ELISA | Assay-dependent | |
| ChIP assay | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around Acetyl-Lys (120) derived from Human Histone H2B type 1-C/E/F/G/I |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.16 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.03% Proclin 300 |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_3093644 |
Target Information
Histones are basic nuclear proteins responsible for nucleosome structure in eukaryotic chromatin. Each nucleosome consists of an octamer of core histones (H2A, H2B, H3, and H4) with approximately 146 bp of DNA wrapped around it. The linker histone H1 interacts with linker DNA between nucleosomes, contributing to chromatin compaction. Histone H2B also exhibits antimicrobial activity. The HIST1H2BC gene encodes a replication-dependent histone variant lacking a polyA tail, instead containing a palindromic termination element, and is located within the large histone gene cluster on chromosome 6.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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