Thermo Fisher Scientific Levo-alpha-Acetylmethadol Polyclonal Antibody

Applications and Tested Dilution

• Application: ELISA
• Tested Dilution: 5–10 µg/mL

Product Specifications

Product Specific Information

• Working Concentration: 1/32K
• Sensitivity: 10 ng/mL LAAM produces 80% inhibition in a competitive ELISA employing Levo-Alpha-Acetylmethadol (LAAM) polyclonal antibody.

Target Information

Levomethadyl acetate is found in individuals who have used or taken this drug. It is a narcotic analgesic with long onset and duration of action, mainly used in the treatment of narcotic dependence.
Opiate receptors (Mu, Kappa, Delta) are G-protein–coupled receptors regulating synaptic transmission via effector proteins. Binding of the opiate stimulates the exchange of GTP for GDP on the G-protein complex, inhibiting adenylate cyclase and decreasing intracellular cAMP. This leads to reduced release of nociceptive neurotransmitters such as substance P, GABA, dopamine, acetylcholine, and noradrenaline. Opioids also inhibit release of vasopressin, somatostatin, insulin, and glucagon. Levomethadyl acetate activates calcium-dependent inwardly rectifying potassium channels (OP1 receptor agonist), causing hyperpolarization and reduced neuronal excitability.
(Source: PubChem, NCBI)

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.