Thermo Fisher Scientific Collagen III Polyclonal Antibody
Collagen III 단백질을 검출하기 위한 Rabbit Polyclonal Antibody로, ELISA, Western blot, IHC 등 다양한 응용에 적합. 인간 및 소 반응성. 비결합형 액상 시약으로 항원 친화 크로마토그래피로 정제됨. 연구용 전용 제품.
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Thermo Fisher Scientific Collagen III Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 | - |
| Immunohistochemistry (Frozen) (IHC-F) | Assay-Dependent | - |
| ELISA | 1:5,000–1:50,000 | - |
| Immunoprecipitation (IP) | 1:100 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Collagen Type III from human and bovine placenta |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.16 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_1956965 |
Product Specific Information
PA1-28870 has been successfully used in ELISA, immunohistochemistry (frozen tissue), immunoprecipitation, and Western blot applications.
Note: The preparation of a specific antibody to Collagen III’s uninterrupted Glycine-X-Y triplet repeat requires a non-denatured epitope format. Consequently, it is recommended to use this antibody in Western blot under non-denaturing or native conditions.
Store as concentrated solution. Centrifuge briefly prior to opening vial.
Target Information
Collagen III is a fibrillar collagen found in extensible connective tissues such as skin, lung, and the vascular system, often in association with type I Collagen.
Mutations in this gene are associated with Ehlers-Danlos syndrome type IV and with aortic and arterial aneurysms.
Although alternate transcripts have been detected for this gene, they result from mutations that alter splicing, often leading to exclusion of multiple exons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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