Thermo Fisher Scientific Collagen III Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

-

ELISA (ELISA)

1:5,000-1:50,000

-

Immunoprecipitation (IP)

1:100

-

Product Specifications

Species Reactivity

Bovine, Human

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Collagen Type III from human and bovine placenta if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Collagen III,uniProtId:P02461-1,ncbiNodeId:9606,antigenRange:1-1466,antigenLength:1466,antigenImageFileName:PA1-28870_Collagen_III_P02461-1_Rabbit.svg,antigenImageFileNamePDP:PA1-28870_Collagen_III_P02461-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:Collagen III,uniProtId:P04258-1,ncbiNodeId:9913,antigenRange:1-1049,antigenLength:1049,antigenImageFileName:PA1-28870_Collagen_III_P04258-1_Rabbit.svg,antigenImageFileNamePDP:PA1-28870_Collagen_III_P04258-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.16 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_1956965

Product Specific Information

PA1-28870 has been successfully used in ELISA, immunohistochemistry (frozen tissue), immunoprecipitation, and Western blot applications. NOTE: The preparation of a specific antibody to Collagen IIIs uninterruptedGlycine-X-Ytriplet repeat requires a non-denatured epitope format. Consequently, it is recommended to use this antibody in a Western blot using non-denaturing ornative` conditions.

Store as concentrated solution. Centrifuge briefly prior to opening vial.

Target Information

Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in th gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.