Thermo Fisher Scientific F(ab`)2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Functional Grade, eBioscience

Applications

Tested Dilution

Flow Cytometry (Flow)

Assay-Dependent

Functional Assay (Functional)

Assay-Dependent

Product Specifications

Host/Isotype

Goat / Ig

Class

Polyclonal

Type

Secondary Antibody

Conjugate

Functional Grade Functional Grade Functional Grade

View additional formats

• [Agarose](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Agarose/host/Goat/)
• [Alexa Fluor 488 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 488/host/Goat/)
• [Alexa Fluor 488-10 nm colloidal gold](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 488-10 nm colloidal gold/host/Goat/)
• [Alexa Fluor 488-FluoroNanogold](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 488-FluoroNanogold/host/Goat/)
• [Alexa Fluor 555 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 555/host/Goat/)
• [Alexa Fluor 594-FluoroNanogold](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 594-FluoroNanogold/host/Goat/)
• [Alexa Fluor 647 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 647/host/Goat/)
• [Alexa Fluor 680 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 680/host/Goat/)
• [Alexa Fluor 790 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 790/host/Goat/)
• [Alexa Fluor Plus 405 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor Plus 405/host/Goat/)
• [Alexa Fluor Plus 488 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor Plus 488/host/Goat/)
• [Alexa Fluor Plus 647 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor Plus 647/host/Goat/)
• [AP](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/AP/host/Goat/)
• [APC ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/APC/host/Goat/)
• [bGal](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/bGal/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Biotin/host/Goat/)
• [DyLight 350](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 350/host/Goat/)
• [DyLight 405](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 405/host/Goat/)
• [DyLight 488](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 488/host/Goat/)
• [DyLight 550](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 550/host/Goat/)
• [DyLight 594](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 594/host/Goat/)
• [DyLight 633](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 633/host/Goat/)
• [DyLight 650](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 650/host/Goat/)
• [DyLight 680](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 680/host/Goat/)
• [DyLight 800 4X PEG](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/DyLight 800 4X PEG/host/Goat/)
• [eFluor 570](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/eFluor 570/host/Goat/)
• [eFluor 660](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/eFluor 660/host/Goat/)
• [FITC](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/FITC/host/Goat/)
• [HRP](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/HRP/host/Goat/)
• [NovaFluor Red 685](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/NovaFluor Red 685/host/Goat/)
• [NovaFluor Red 700](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/NovaFluor Red 700/host/Goat/)
• [NovaFluor Yellow 590](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/NovaFluor Yellow 590/host/Goat/)
• [NovaFluor Yellow 610](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/NovaFluor Yellow 610/host/Goat/)
• [NovaFluor Yellow 660](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/NovaFluor Yellow 660/host/Goat/)
• [PE ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/PE/host/Goat/)
• [PerCP-eFluor 710](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/PerCP-eFluor 710/host/Goat/)
• [Qdot 525](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 525/host/Goat/)
• [Qdot 565](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 565/host/Goat/)
• [Qdot 585](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 585/host/Goat/)
• [Qdot 605](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 605/host/Goat/)
• [Qdot 655](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 655/host/Goat/)
• [Qdot 705](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 705/host/Goat/)
• [Qdot 800](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 800/host/Goat/)
• [Rhodamine](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Rhodamine/host/Goat/)
• [TRITC](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/TRITC/host/Goat/)
• [WesternDot 625](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/WesternDot 625/host/Goat/)
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2865998

Target

IgG

Antibody Form

F(ab`)2 Fragment

Product Specific Information

Description: This polyclonal F(ab`)2 fragment recognizes mouse IgG. Reactivity to human, bovine, and horse has been removed. This polyclonal antibody can be used to crosslink and activate B lymphocytes.

Applications Reported: This polyclonal F(ab`)2 Goat Anti-Mouse IgG has been reported for use in functional assays (such as B cell activation). Concentrations should be determined empirically for optimal performance in the assay of interest.

Applications Tested: F(ab`)2 Anti-Mouse IgG Functional Grade Purified has been tested by in vitro stimulation of mouse splenocytes.

Endotoxin: Less than 0.1 ng/µg antibody as determined by the LAL assay.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.