Thermo Fisher Scientific B7-H6 Chimeric Recombinant Rabbit Monoclonal Antibody (17B1.3)
Thermo Fisher Scientific의 B7-H6 Chimeric Recombinant Rabbit Monoclonal Antibody (17B1.3)는 인간 B7-H6 특이 항체로, ICC/IF, Flow Cytometry 및 In vitro Assay에 적합합니다. NKp30 결합을 방해하지 않으며 NK 세포 활성화를 억제합니다. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 1:100 |
| In vitro Assay (IV) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 17B1.3 |
| Immunogen | Immunised mice with extracellular domain of human B7-H6 and selected on the basis of specific reactivity by P815.B7-H6, but not with P815.B7-H1. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074729 |
Product Specific Information
Specificity: mAb 17B1.3 specifically recognises B7-H6.
17B1.3 reacted with human tumour cell lines constitutively expressing B7-H6 such as HeLa cells.
Fab 17B1.3 does not interfere with the binding of B7-H6 to NKp30, but nevertheless blocks NK cell activation.
Target Information
B7-H6 (NCR3LG1) is a transmembrane endogenous ligand expressed on the surfaces of tumor cells.
B7-H6 binds with NKp30 present on NK cells. Binding of B7-H6 to NKp30 causes ligation of NKp30, subsequently inducing NK cell activation and target cell cytolysis.
B7-H6 is not constitutively expressed on normal tissue. Whether it can be expressed endogenously under certain conditions is under investigation.
B7-H6 has been detected on circulating pro-inflammatory CD14(+)CD16(+) monocytes in some patients with sepsis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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