Thermo Fisher Scientific B7-H6 Chimeric Recombinant Rabbit Monoclonal Antibody (17B1.3)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

10 µg/mL

-

Flow Cytometry (Flow)

1:100

-

In vitro Assay (IV)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

17B1.3

Immunogen

Immunised mice with extracellular domain of human B7-H6 and selected on the basis of specific reactivity by P815.B7-H6, but not with P815.B7-H1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074729

Product Specific Information

Specificity: mAb 17B1.3 specifically recognises B7-H6. 17B1.3 reacted with human tumour cell lines constitutively expressing B7-H6 such as HeLa cells. Fab 17B1.3 does not interfere with the binding of B7-H6 to NKp30, but nevertheless blocks NK cell activation.

Target Information

B7-H6 (NCR3LG1) is a transmembrane endogenous ligand expressed on the surfaces of tumor cells. B7-H6 binds with NKp30 present on NK cells. Binding of B7-H6 to NKp30 causes the ligation of NKp30 subsequently inducing NK cell activation and target cell cytolysis. B7-H6 is not constitutively expressed on normal tissue. Whether it can be expressed endogenously under certain conditions is under investigation. B7-H6 has been detected on circulating pro-inflammatory CD14(+)CD16(+) monocytes in some patients with sepsis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.