Thermo Fisher Scientific Thomsen-Friedenreich Antigen/CD176 (Pan Carcinoma Marker) Monoclonal Antibody (SPM320)
Thomsen-Friedenreich Antigen/CD176을 인식하는 SPM320 단클론 항체로, 다양한 암종에서 발현되는 Pan Carcinoma Marker로 사용. IHC 및 ICC/IF에 적합하며, 인간·마우스·랫트 반응성. 단클론 Mouse IgM 항체로 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 4 µg/mL |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 2–4 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM320 |
| Immunogen | Neuraminidase-treated human red blood cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The Thomsen-Friedenreich antigen acts as an oncofetal antigen, with low expression in normal adult tissues but increasing to fetal levels of expression in hyperplasia or malignancy. It is considered a pan-carcinoma marker. This gene belongs to the mucin family and encodes a membrane-bound, glycosylated phosphoprotein anchored to the apical surface of many epithelia via a transmembrane domain. The degree of glycosylation varies with cell type and includes a 20 amino acid variable number tandem repeat (VNTR) domain, with repeat numbers ranging from 20 to 120 among individuals. The protein serves protective and signaling functions, and its overexpression or altered glycosylation has been associated with carcinomas. Multiple alternatively spliced transcript variants exist, though only some full-length forms are confirmed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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