Triple Sugar Iron Agar
Details
Used for the identification of gram-negative enteric bacilli on the basis of dextrose, lactose and sucrose fermentation and hydrogen sulphide production. Recommended by BIS for detection of Escherichia coli and Vibrio. The reactions can be interpreted as follows: Alkaline slant / acid butt- only glucose fermented, Acid slant / acid butt- dextrose and sucrose fermented or dextrose and lactose fermented or all the three sugars, dextrose,lactose and sucrose fermented, Bubbles or cracks present-gas production Black precipitate present-H2S gas production
