Thermo Fisher Scientific IL-12 p35 Monoclonal Antibody (27537), FITC
IL-12 p35 단백질을 인식하는 FITC 결합 단클론 항체로, 인간 및 마우스 시료에 반응합니다. Flow Cytometry에 적합하며, p35 단위체 또는 p40/p35 이합체를 검출합니다. Protein A/G 정제, 4°C 보관, 형광 최대치 498/517 nm.
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Applications
- Flow Cytometry (Flow): 10 µL per million cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 27537 |
| Immunogen | Sf21-derived recombinant human IL-12/IL-35 p35 (Arg23–Ser219) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | Buffered saline solution with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in the dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609033 |
Additional Formats
- PE (Cat. MA5-23559)
- PerCP (Cat. MA5-23622)
- Custom conjugation available upon request
Product Specific Information
This antibody detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. It does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with p35 subunits from porcine and mouse systems.
Target Information
Interleukin 12A (IL-12A) is a cytokine belonging to the Interleukin-6 protein superfamily. The IL-12A alpha chain precursor is a 35-kDa subunit of the disulfide-linked heterodimer IL-12 and exhibits diverse biological activities. IL-12A acts as a growth factor for activated T and NK cells, enhances NK cell cytotoxicity, and stimulates IFN-gamma production. It also contributes to Th1 and Th2 cell differentiation and regulates Stat4 protein phosphorylation. Together with EBI3, IL-12A forms IL-35, a cytokine associated with Treg-mediated immunosuppressive activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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