Thermo Fisher Scientific MRPL12 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human MRPL12. Recombinant protein control fragment (Product #RP-93518). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MRPL12,uniProtId:P52815-1,ncbiNodeId:9606,antigenRange:103-198,antigenLength:198,antigenImageFileName:PA5-54621_MRPL12_P52815-1_Rabbit.svg,antigenImageFileNamePDP:PA5-54621_MRPL12_P52815-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2644119

Product Specific Information

Immunogen sequence: VMSGAVPAAA AQEAVEEDIP IAKERTHFTV RLTEAKPVDK VKLIKEIKNY IQGINLVQAK KLVESLPQEI KANVAKAEAE KIKAALEAVG GTVVLE

Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 86%.

Target Information

MRPL12 (mitochondrial ribosomal protein L12), also referred to as 5c5-2, L12mt, MRPL7 or RPML12, is a mammalian mitochondrial ribosomal protein that is involved in protein synthesis within the mitochondrion. MRPL12 is enhanced in growth-stimulated cells as a result of transcriptional activation, suggesting that it may function as a translational regulator of mitochondrial mRNAs. Impairment of MRPL12 leads to reduction in cell growth rate, decreased mitochondrial ATP production and abolition of mitochondrial oxidative phosphorylation. MRPL12 is cleaved during its translocation across the mitochondrial membrane and it exists as dimers that bind the large ribosomal subunit. MRPL12 is 198 amino acids in length, belongs to the ribosomal protein L12P family and is highly expressed in the colon.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.