Thermo Fisher Scientific MRPL12 Polyclonal Antibody
Human MRPL12 단백질을 표적으로 하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS 기반의 안정적인 저장 버퍼를 사용합니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 | - |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human MRPL12. Recombinant protein control fragment (Product #RP-93518). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2644119 |
Product Specific Information
Immunogen sequence:
VMSGAVPAAA AQEAVEEDIP IAKERTHFTV RLTEAKPVDK VKLIKEIKNY IQGINLVQAK KLVESLPQEI KANVAKAEAE KIKAALEAVG GTVVLE
Highest antigen sequence identity to the following orthologs:
- Mouse: 86%
- Rat: 86%
Target Information
MRPL12 (mitochondrial ribosomal protein L12)
Also known as 5c5-2, L12mt, MRPL7, or RPML12. MRPL12 is a mammalian mitochondrial ribosomal protein involved in protein synthesis within the mitochondrion. It is enhanced in growth-stimulated cells due to transcriptional activation, suggesting a role as a translational regulator of mitochondrial mRNAs.
Impairment of MRPL12 leads to reduced cell growth, decreased mitochondrial ATP production, and loss of oxidative phosphorylation. MRPL12 exists as dimers that bind the large ribosomal subunit, consists of 198 amino acids, belongs to the ribosomal protein L12P family, and is highly expressed in the colon.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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