Thermo Fisher Scientific ACOT2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human ACOT2(Accession P49753), corresponding to amino acid residues D177-G227. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ACOT2,uniProtId:P49753-1,ncbiNodeId:9606,antigenRange:177-227,antigenLength:483,antigenImageFileName:PA5-101403_ACOT2_P49753-1_Rabbit.svg,antigenImageFileNamePDP:PA5-101403_ACOT2_P49753-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2850839

Product Specific Information

Antibody detects endogenous levels of total ACOT2.

Target Information

Acyl-CoA thioesterases (ACOTs) are a group of enzymes that catalyze the hydrolysis of acyl-CoA to form coenzyme A (CoA) and a free fatty acid. Through their catalytic activity, ACOTs are able to regulate the level of fatty acids and acyl-CoAs within the cell. ACOT1 (acyl-CoA thioesterase 1, also known as CTE1) and ACOT2 (acyl-CoA thioesterase 2, also known as PTE2) are members of the ACOT family and exhibit different cellular localization, with ACOT1 existing as a monomer in the cytoplasm and ACOT2 localized primarily to mitochondria. Characteristic of most ACOT proteins, ACOT1 and ACOT2 catalyze the conversion of Palmitoyl-CoA and water to free CoA and palmitate, a reaction that is important for the regulation of intercellular fatty acid levels. ACOT2 is expressed as multiple alternatively spliced isoforms and, like ACOT1, is encoded by a gene which maps to human chromosome 14.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.