Thermo Fisher Scientific Phospho-c-Kit (Tyr719) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT and its ligand regulate the growth and activation of various hematopoietic and non-hematopoietic cells.

Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Deregulation of the KIT receptor tyrosine kinase, such as the D816V mutation, is a key focus in therapeutic strategies targeting neoplastic mast cell growth.

c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and gastrointestinal tract Cajal cells. Upon binding of its ligand stem cell factor (SCF), c-Kit dimerizes, leading to receptor activation and autophosphorylation of tyrosine residues including Tyr703. This modification enables docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-Kit can also activate PI3K, PLC-γ, and JAK/STAT pathways. c-Kit receptor activation is essential for hematopoiesis, stem cell maintenance, and gametogenesis.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.