Thermo Fisher Scientific Dog BSEP Vesicles

Gibco™ Dog BSEP Vesicles

Dog BSEP Vesicles are insect-derived purified plasma membranes with inserted BSEP transport protein (bile salt export pump, ABCB11). These vesicles can be used to evaluate test compounds and drug interactions with BSEP in in vitro assays. BSEP is an ATP-binding cassette (ABC) transporter that plays a key role in bile acid excretion from the liver.

Use Dog BSEP Vesicles to

• Investigate transporter interactions of drug candidates
• Assess potential for transporter-mediated drug-drug interactions
• Obtain high-quality results with large signal-to-noise ratios

Concept Behind ABC Transporter Vesicles

ABC transporter vesicles are easy-to-use, efficient reagents for early assessment of a drug candidate’s substrate and drug interaction potential. While ABC transporters typically mediate the export of substrates out of cells, transporters expressed on these inside-out vesicles import substrates into the vesicles. This allows quantitative evaluation of transport activity by measuring the amount incorporated into the vesicles.

Clear and Reliable Results

Prepared from Sf9 cells engineered to overexpress specific ABC transporters, these inside-out vesicles provide high levels of transporter activity with low background, enabling clear detection of whether a compound is a substrate or inhibitor of a specific efflux transporter.

Transporter Vesicle Details

Dog BSEP Vesicles are part of the ABC transporter vesicle group. They are prepared from purified plasma membranes isolated from an insect cell system (Sf9 cells transfected with baculovirus) expressing BSEP. Evaluation of inhibitory activity of new compounds toward BSEP-mediated transport of bile acids (e.g., taurocholate) may indicate potential hepatotoxicity.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

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