Thermo Fisher Scientific Phospho-Smad3 (Thr179) Polyclonal Antibody
Phospho-Smad3 (Thr179) 폴리클로날 항체로 인간, 마우스, 랫트에 반응하며 다양한 응용(서던 블롯, IHC, ICC, Flow, ELISA)에 적합. Protein A로 정제된 고순도 항체로, -20°C에서 안정적으로 보관 가능. 연구용으로만 사용.
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Thermo Fisher Scientific Phospho-Smad3 (Thr179) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of Thr179 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
SMAD3 belongs to the SMAD family of proteins, which are homologous to the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma. SMAD proteins act as signal transducers and transcriptional modulators mediating multiple signaling pathways. SMAD3 functions as a transcriptional modulator activated by transforming growth factor-beta (TGF-β) and plays a role in the regulation of carcinogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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