Thermo Fisher Scientific SIK3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000-1:2,500

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human SIK3. Recombinant protein control fragment (Product #RP-100419). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SIK3,uniProtId:Q9Y2K2-5,ncbiNodeId:9606,antigenRange:604-703,antigenLength:1321,antigenImageFileName:PA5-60959_SIK3_Q9Y2K2-5_Rabbit.svg,antigenImageFileNamePDP:PA5-60959_SIK3_Q9Y2K2-5_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2647304

Product Specific Information

Immunogen sequence: STYKDSNTLH LPTERFSPVR RFSDGAASIQ AFKAHLEKMG NNSSIKQLQQ ECEQLQKMYG GQIDERTLEK TQQQHMLYQQ EQHHQILQQQ IQDSICPPQP

Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 97%.

Target Information

SIK3 (QSK) is a serine/threonine-protein kinase, belongs to QIK subfamily. The phosphorylation of SIK3 by LKB1 through the 14-3-3 binding enhances its catalytic activity and leads its localization to punctate structures within the cytoplasm. Overexpression of SIK3 promotes G1/S cell cycle progression with ovarian cancer. There are two sites (H331L and A1103V) were mutated at significant frequency in breast cancer. SIK3 is a novel tumor-associated antigen (TAA). SIK3 is a positive regulator of mTOR signaling that functions by triggering the degradation of DEPTOR, an mTOR inhibitor. Involved in the dynamic regulation of mTOR signaling in chondrocyte differentiation during skeletogenesis. Negatively regulates cAMP signaling pathway possibly by acting on CRTC2/TORC2 and CRTC3/TORC3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.