Thermo Fisher Scientific Zymosan A (S. cerevisiae) BioParticles, Alexa Fluor 488 conjugate

Invitrogen™ Zymosan A (S. cerevisiae) BioParticles™, Alexa Fluor™ 488 Conjugate

The Molecular Probes™ BioParticles™ product line consists of fluorescently labeled, heat- or chemically killed bacteria and yeast in a variety of sizes, shapes, and natural antigenicities. These fluorescent BioParticles™ products are widely used to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry.

We offer E. coli (K-12 strain), S. aureus (Wood strain, without protein A), and zymosan (S. cerevisiae) BioParticles™ conjugates covalently labeled with various fluorophores. Free dye is carefully removed after conjugation. Unlike fluorescein-labeled conjugates whose fluorescence can be quenched in acidic environments, Alexa Fluor™, BODIPY™ FL, tetramethylrhodamine, and Texas Red™ conjugates maintain strong fluorescence across pH 3–10.

BioParticles Specifications

Using BioParticles™ Products

BioParticles™ conjugates are supplied as lyophilized powders.

• Approximately 3 × 10⁸ E. coli or S. aureus particles per mg solid
• Approximately 2 × 10⁷ zymosan particles per mg solid

These conjugates can be reconstituted in a buffer of choice for phagocytosis assays. Surface-bound (non-internalized) BioParticles™ fluorescence can be quenched using ethidium bromide, trypan blue, or similar quenchers.

In addition to cellular applications, fluorescent BioParticles™ conjugates can serve as calibration references for flow cytometry or as light scattering standards due to their characteristic size and shape differences.

Related Products

• A wide range of dye-labeled and unlabeled BioParticles™ (E. coli, S. aureus, zymosan) are available.
• For detailed application information, refer to the Molecular Probes™ Handbook, Section 16.1.
• For pH-sensitive endocytosis assays, see pHrodo™ BioParticles™ conjugates.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

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