Thermo Fisher Scientific BLOCK-iT Transfection Optimization Kit

Invitrogen™ BLOCK-iT™ Transfection Optimization Kit

The BLOCK-iT™ Transfection Optimization Kit is designed to optimize RNAi transfection using controls for transfection and viability.

Each kit contains a BLOCK-iT™ Fluorescent Oligo, which provides:

• A strong, easily detectable FITC signal indicating transfection efficiency
• Chemical modifications for a clearer, more persistent cellular signal than labeled siRNA provides
• Proven correlation of transfection efficiency with Stealth RNAi™ siRNA and traditional siRNA molecules

This makes BLOCK-iT™ Fluorescent Oligo an ideal indicator of transfection efficiency for RNAi experiments.

In addition, the kit contains:

• A Positive Control Stealth RNAi™ siRNA molecule directed at human p53
• A Scrambled Stealth RNAi™ siRNA molecule as a negative control
• Dead Cell Dye, which stains dead cells red for easy identification of non-viable cells in tissue culture plates

Using these reagents together allows you to both optimize transfection conditions and have a functional positive control in every RNAi experiment.

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