Thermo Fisher Scientific A-RAF Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–10 µg/mg lysate

Product Specifications

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

Several serine/threonine protein kinases act as intermediates in signal transduction pathways, including ERK/MAP kinases, ribosomal S6 kinase (Rsk), and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine kinase activity and is the cellular homolog of v-Raf, the transforming gene product of the 3611 strain of murine sarcoma virus. Unregulated kinase activity of v-Raf is linked to transformation and mitogenesis, while Raf-1 activity is normally suppressed by a regulatory N-terminal domain.
A-Raf, another member of the Raf gene family, shows high homology to Raf-1 within the kinase domain but less elsewhere. A-Raf expression is highest in urogenital tissues and kidney and lowest in brain tissue.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.