Thermo Fisher Scientific POLR2A Polyclonal Antibody

Applications

Immunocytochemistry (ICC/IF)

• Tested Dilution: 0.25–2 µg/mL

Product Specifications

Product Specific Information

Immunogen sequence:
GFGDDLNCIF NDDNAEKLVL RIRIMNSDEN KMQEEEEVVD KMDDDVFLRC IESNMLTDMT LQGIEQISKV YMHLPQTDNK KKIIITEDGE FKALQEWILE TDGVSLMRVL S

Target Information

DNA-directed RNA polymerase II subunit RPB1 (POLR2A) is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
POLR2A is the largest subunit and a catalytic component of RNA polymerase II, which synthesizes mRNA precursors and many functional non-coding RNAs. It forms the polymerase active center together with the second largest subunit.

Pol II is the central component of the basal RNA polymerase II transcription machinery, composed of mobile elements that move relative to each other. RPB1 contributes to the core element with the central large cleft, the clamp element that opens and closes the cleft, and the jaws that engage the incoming DNA template.

At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix from RPB1 crosses the cleft near the catalytic site and promotes translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site via conformational changes.

During transcription elongation, Pol II moves along the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of RPB1, which serves as a platform for assembly of factors regulating transcription initiation, elongation, termination, and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of CTD lysines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.