Thermo Fisher Scientific Phospho-Vinculin (Tyr821) Polyclonal Antibody
Rabbit polyclonal antibody recognizing phospho-Vinculin (Tyr821). Suitable for IHC(P) and ICC/IF applications. Supplied as a 1 mg/mL liquid, unconjugated form. Targets human Vinculin involved in cell adhesion and cytoskeletal organization.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic phosphopeptide derived from human Vinculin around the phosphorylation site of Tyr821 (S-G-YP-R-I) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2555145 |
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell–cell and cell–extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane.
A sequence of molecular interactions is involved in the transmembrane assembly of adhesion plaques: the beta subunit of integrin binds to talin, talin binds to vinculin, and vinculin interacts with alpha-actinin and possibly itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in linking the extracellular matrix to the cytoplasmic microfilament system.
Mutations in the VCL gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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