Thermo Fisher Scientific Bu-1a Monoclonal Antibody (21-1A4), FITC
닭 Bu-1a 항원을 인식하는 FITC 결합 단클론 항체로, Flow Cytometry에 적합합니다. 498/517 nm 형광 특성을 가지며, 0.5 mg/mL 농도의 액상 형태로 제공됩니다. PBS(pH 7.4) 버퍼에 보관하며, 4°C 암소 보관이 권장됩니다.
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Applications
- Flow Cytometry (Flow): 1 µg/10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 21-1A4 |
| Immunogen | Bursa cells from one-day-old H.B15 (Bu-1a/b) strain chickens |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4 |
| Contains | <0.1% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745712 |
Additional Formats
- Unconjugated (Cat. No. MA5-28755)
- Biotin (Cat. No. MA5-28752)
- PE (Cat. No. MA5-28754)
- Custom conjugation available upon request
Target Information
Bu-1, a product of the Bu-1a and Bu-1b alleles, is a type I transmembrane glycosylated disulfide-linked homodimer. The monoclonal antibody AV20 recognizes a monomorphic determinant on the Bu-1 B cell-associated alloantigens of both RPL 6(3) (Bu-1a) and 7(2) (Bu-1b) lines of inbred chickens. The antibody also identifies B cells in partially inbred birds. Bu-1 is found on 85–90% of bursal cells, 2–8% of thymocytes, 15–27% of spleen cells, and 2–18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes but not on granulocytes, erythrocytes, or thrombocytes. The monoclonal antibody AV20 does not cross-react with turkey cells when analyzed by flow cytometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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