Thermo Fisher Scientific RAP80 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000-1:25,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000 to 1:5,000

-

Immunoprecipitation (IP)

2 -10 µg/mg lysate

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 650 and the C-terminus (residue 719) of Human Receptor Associated Protein 80. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RAP80,uniProtId:Q96RL1-1,ncbiNodeId:9606,antigenRange:650-719,antigenLength:719,antigenImageFileName:A300-764A-M_RAP80_Q96RL1-1_Rabbit.svg,antigenImageFileNamePDP:A300-764A-M_RAP80_Q96RL1-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Target Information

RAP80 was initially identified as zinc-finger containing nuclear protein that is highly expressed in testis and interacts with the retinoid-related testis-associated receptor (RTR). Later experiments revealed that RAP80 is recruited by the Coiled-coil domain 98 (CCDC98) protein to the breast cancer-1 protein BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both RAP80 and CCDC98 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either RAP80 or CCDC98 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that RAP80 and CCDC98 control only part of the DNA damage response role of BRCA1. At least four isoforms of RAP80 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.