Thermo Fisher Scientific ARHGEF7 Polyclonal Antibody
ARHGEF7 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot 및 IHC(P) 검증 완료. 인간, 마우스, 랫에서 반응하며, 액상 형태로 제공. 친화 크로마토그래피로 정제되어 높은 특이성과 재현성 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ARHGEF7 (Accession Q14155), corresponding to amino acid residues N311–Y361 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2851281 |
Product Specific Information
Antibody detects endogenous levels of total ARHGEF7.
Target Information
The serine/threonine kinase p21 activated kinase (PAK) is a downstream effector of the small GTPases Cdc42 and Rac. PAK associates with Nck, the p85 and p110 subunits of PI3-kinase, and PIX (PAK-interacting exchange factor) in a focal complex. Binding of PIX is necessary for localization and activation of PAK in the Cdc42 to Rac signaling pathway, mediated through the high affinity of the N-terminal SH3 domain of PIX for a conserved proline-rich PAK sequence.
PIX exists as two isoforms, alpha and beta, both highly expressed in heart, muscle, and thymus tissues of human and rat. The alphaPIX isoform acts as a guanine nucleotide exchange factor (GEF) on Rac, mediating lamellipodia formation and is phosphorylated via PDGF and EphB2 receptor signaling pathways or through association with PI3-kinase.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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