Thermo Fisher Scientific USP13 Polyclonal Antibody

Thermo Fisher Scientific USP13 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Target Information

Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2, and USP10, involved in processes like autophagy and endoplasmic reticulum-associated degradation (ERAD).
Component of a regulatory loop controlling autophagy and p53/TP53 levels: stabilizes PIK3C3/VPS34-containing complexes by deubiquitinating BECN1, a key autophagy regulator. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability.
PIK3C3/VPS34-containing complexes, in turn, regulate USP13 stability, suggesting a feedback system managing p53/TP53 protein levels via USP10 and USP13.
Recruited by nuclear UFD1, USP13 mediates deubiquitination of SKP2, regulating ERAD. It also acts on UBL4A, a component of the BAG6/BAT3 complex.
USP13 stabilizes SIAH2 independently of deubiquitinase activity by binding ubiquitinated SIAH2 and inhibiting its autoubiquitination.
Shows weak deubiquitinase activity in vitro, preferentially cleaving Lys-63-linked polyubiquitin chains. Unlike USP5, it does not disassemble unanchored polyubiquitin.
Can cleave ISG15 in vitro, though further validation is required. [UniProt]

Usage Notes

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.