Invitrogen™

SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA자세히 알아보기

SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR. This system combines RT/Platinum Taq mix and 2X reaction mix. The RT/Platinum Taq mix contains a mixture of SuperScript II Reverse Transcriptase and Platinum Taq DNA Polymerase. The 2X reaction mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg2+ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers.

All components for cDNA synthesis and PCR are combined in a single tube with gene-specific primers and target RNA. Reverse transcription automatically follows PCR cycling without additional steps.

Note: For superior one-step RT-PCR performance, we recommend the SuperScript IV One-Step RT-PCR System or SuperScript IV One-Step RT-PCR System with ezDNase. The SuperScript IV One-Step RT-PCR System combines the high processivity of SuperScript IV Reverse Transcriptase with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched product yields, specificity, and sensitivity in less time and for a broad target range, even with suboptimally pure RNA samples.

사양

검출 방법Gel Electrophoresis

형식Kit

PCR 방법1-step RT-PCR

중합효소Platinum Taq DNA Polymerase

반응 속도30 to 50 min.

기술1-step RT-PCR

최적 반응 온도55°C to 60°C

수량100 rxns

역전사 효소SuperScript II

리보뉴클레아제 H 활성Reduced

배송 조건Wet Ice

최종 제품 유형PCR Amplified cDNA

핫 스타트Built-In Hot Start

반응 수100 Reactions

반응 형식Master Mix

시약 유형Reverse Transcription

크기(최종 제품)Up to 7 kb

시작 물질RNA

Unit SizeEach