Thermo Fisher Scientific EpCAM Monoclonal Antibody (323/A3)

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:10-1:200

View 6 publications 6 publications

Flow Cytometry (Flow)

0.5-1 µg/test

View 2 publications 2 publications

Radioimmune Assays (RIA)

Assay-dependent

-

Affinity Purification (AP)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

323/A3

Immunogen

MCF-7 human breast cancer cells if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EpCAM (CD326),uniProtId:P16422-1,ncbiNodeId:9606,antigenRange:1-314,antigenLength:314,antigenImageFileName:MA5-12436_EpCAM_CD326_P16422-1_House_mouse.svg,antigenImageFileNamePDP:MA5-12436_EpCAM_CD326_P16422-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10981962

Product Specific Information

MA5-12436 targets Epithelial Specific Antigen in FACS, ICC/IF, IHC (P), and RIA applications and shows reactivity with Human and Mouse samples.

The MA5-12436 immunogen is mCF-7 human breast cancer cells.

Target Information

Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types. Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.