Thermo Fisher Scientific ViralSEQ Mouse Minute Virus (MMV) Detection System

Applied Biosystems™ ViralSEQ™ Mouse Minute Virus (MMV) Detection System

The Applied Biosystems ViralSEQ Mouse Minute Virus (MMV) Detection Assay is part of an integrated DNA purification and real-time PCR-based system for rapid, sensitive detection of MMV from a wide range of sample types. Viral DNA is isolated using the PrepSEQ 1-2-3 Nucleic Acid Extraction Kit (available separately). Detection is performed using TaqMan real-time PCR technology with a proprietary positive control molecule that eliminates false positives from contamination.

High Specificity and Rapid Results

• Detects four known strains of MMV: i, p, m, and c (seven genomic sequences in NCBI database)
• No cross-reactivity with unrelated DNA
• Simple workflow delivers results in under 4 hours

Streamlined Process for Surveillance and Early Detection

Part of the Cell Culture Rapid Methods Program, designed to detect common contaminants (mycoplasma, vesivirus, MMV) in mammalian cell culture-based biopharmaceutical manufacturing. Combines single sample preparation with real-time PCR assays on one instrument platform.

Reliable Results

Includes MMV Discriminatory Positive/Extraction Control, a plasmid DNA containing MMV sequence recognized by the FAM probe.

• Control behaves like MMV DNA during preparation and detection
• Engineered with VIC probe binding sequence to distinguish true MMV positives (FAM only) from control DNA (FAM + VIC)
• Prevents false positives due to cross-contamination

Low-Level Detection

Uses TaqMan technology for highly sensitive detection down to 10 copies of MMV genomic DNA per PCR reaction (≈0.002 TCID50). Demonstrates broad dynamic range and efficient amplification.

Superior Sample Preparation

The PrepSEQ 1-2-3 Nucleic Acid Extraction Kit (Cat. No. 4452222) ensures consistent and high recovery of MMV DNA from complex samples (e.g., CHO cells, bovine serum). Ct values from spiked samples are comparable to unspiked controls, confirming efficient DNA recovery.

사양 (Specifications)

제품 이미지

(이미지들은 본문 하단에 통합되어 표시됩니다)