Thermo Fisher Scientific ViralSEQ Mouse Minute Virus (MMV) Detection System
MMV 검출을 위한 고감도 qPCR 기반 시스템으로, 4시간 이내 결과 확인 가능. TaqMan 기술을 사용해 높은 특이성과 민감도(≥10 copies MMV DNA)를 제공. PrepSEQ 1-2-3 키트와 연계해 다양한 시료에서 안정적 DNA 추출 가능. 오염 방지를 위한 독자적 양성/추출 컨트롤 포함.
- 카탈로그번호
- 4444415
- 판매단위
- pk
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Applied Biosystems™ ViralSEQ™ Mouse Minute Virus (MMV) Detection System
The Applied Biosystems ViralSEQ Mouse Minute Virus (MMV) Detection Assay is part of an integrated DNA purification and real-time PCR-based system for rapid, sensitive detection of MMV from a wide range of sample types. Viral DNA is isolated using the PrepSEQ 1-2-3 Nucleic Acid Extraction Kit (available separately). Detection is performed using TaqMan real-time PCR technology with a proprietary positive control molecule that eliminates false positives from contamination.
High Specificity and Rapid Results
- Detects four known strains of MMV: i, p, m, and c (seven genomic sequences in NCBI database)
- No cross-reactivity with unrelated DNA
- Simple workflow delivers results in under 4 hours
Streamlined Process for Surveillance and Early Detection
Part of the Cell Culture Rapid Methods Program, designed to detect common contaminants (mycoplasma, vesivirus, MMV) in mammalian cell culture-based biopharmaceutical manufacturing. Combines single sample preparation with real-time PCR assays on one instrument platform.
Reliable Results
Includes MMV Discriminatory Positive/Extraction Control, a plasmid DNA containing MMV sequence recognized by the FAM probe.
- Control behaves like MMV DNA during preparation and detection
- Engineered with VIC probe binding sequence to distinguish true MMV positives (FAM only) from control DNA (FAM + VIC)
- Prevents false positives due to cross-contamination
Low-Level Detection
Uses TaqMan technology for highly sensitive detection down to 10 copies of MMV genomic DNA per PCR reaction (≈0.002 TCID50). Demonstrates broad dynamic range and efficient amplification.
Superior Sample Preparation
The PrepSEQ 1-2-3 Nucleic Acid Extraction Kit (Cat. No. 4452222) ensures consistent and high recovery of MMV DNA from complex samples (e.g., CHO cells, bovine serum). Ct values from spiked samples are comparable to unspiked controls, confirming efficient DNA recovery.
사양 (Specifications)
| 항목 | 내용 |
|---|---|
| 검출 방법 | Primer-probe |
| GC-Rich PCR Performance | High |
| PCR 방법 | qPCR |
| 반응 속도 | Standard |
| 제품 라인 | ViralSEQ™ |
| 제품 유형 | Mouse Minute Virus Detection System |
| 수량 | 100 reactions |
| 민감도 | ≥ 10 copies MMV DNA |
| 배송 조건 | Dry Ice |
| 종 | Mouse |
| 타겟 유기체 분류 | Mouse Minute Virus (MMV) |
| 타겟 특이도 | Target-Specific |
| 반응 수 | 100 Reactions |
| 샘플 종류 | Viral DNA |
| Unit Size | Each |
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