Thermo Fisher Scientific Calcium Orange, AM, cell permeant - Special Packaging
Calcium Orange™, AM은 세포 투과성이 높은 형광 칼슘 지시자로, Ca2+ 결합 시 형광이 약 3배 증가합니다. 세포 내 칼슘 측정, 유입·방출 모니터링, 생조직 다광자 이미징 등에 적합하며, 형광 현미경·플레이트 분석·유세포 분석에 사용됩니다.
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Invitrogen™ Calcium Orange™, AM, cell permeant - Special Packaging
Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca²⁺. They are widely used in calcium signaling investigations, including:
- Measuring intracellular Ca²⁺ levels
- Monitoring Ca²⁺ influx and release
- Multiphoton excitation imaging of Ca²⁺ in living tissues
Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells. The fluorescence signal is typically measured using fluorescence microscopy, microplate assays, or flow cytometry.
Calcium Indicator (AM Ester) Specifications
| 항목 | 내용 |
|---|---|
| Label (Ex/Em) | Calcium Orange™ (549/576 nm) |
| Fluorescence intensity increase upon Ca²⁺ binding | ~3 fold |
| Fluorescence wavelength shift | Minimal upon Ca²⁺ binding |
Spectral Characteristics of Molecular Probes™ Calcium Indicators
These probes are excited by visible light, minimizing cellular photodamage. They are efficiently excited by laser-based instruments such as confocal microscopes, and their emissions occur in spectral regions with reduced autofluorescence and scattering background.
More Choices for Fluorescent Calcium Indicators
Thermo Fisher Scientific offers a wide range of Molecular Probes™ calcium indicators suitable for various experimental needs:
- Dextran versions for reduced leakage and compartmentalization
- BAPTA conjugates for detecting high-amplitude calcium transients
For more information, refer to:
- Fluorescent Ca²⁺ Indicators Excited with Visible Light—Section 19.3
- Indicators for Ca²⁺, Mg²⁺, Zn²⁺ and Other Metal Ions—Chapter 19
For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
제품 사양
| 항목 | 내용 |
|---|---|
| 검출 방법 | Fluorescence |
| 용도(애플리케이션) | Cell Viability and Proliferation |
| 사용 장비 | Fluorescence Microscope |
| 제품 유형 | Calcium Indicator |
| 염료 유형 | Fluorescent Dye-Based |
| 제품 라인 | CALCIUM ORANGE™ |
| 수량 | 10 × 50 μg |
| 배송 조건 | Room Temperature |
| Unit Size | Each |
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