Thermo Fisher Scientific Calcium Orange, AM, cell permeant - Special Packaging

Invitrogen™ Calcium Orange™, AM, cell permeant - Special Packaging

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca²⁺. They are widely used in calcium signaling investigations, including:

• Measuring intracellular Ca²⁺ levels
• Monitoring Ca²⁺ influx and release
• Multiphoton excitation imaging of Ca²⁺ in living tissues

Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells. The fluorescence signal is typically measured using fluorescence microscopy, microplate assays, or flow cytometry.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

Calcium Indicator (AM Ester) Specifications

Spectral Characteristics of Molecular Probes™ Calcium Indicators

These probes are excited by visible light, minimizing cellular photodamage. They are efficiently excited by laser-based instruments such as confocal microscopes, and their emissions occur in spectral regions with reduced autofluorescence and scattering background.

More Choices for Fluorescent Calcium Indicators

Thermo Fisher Scientific offers a wide range of Molecular Probes™ calcium indicators suitable for various experimental needs:

• Dextran versions for reduced leakage and compartmentalization
• BAPTA conjugates for detecting high-amplitude calcium transients

For more information, refer to:

• Fluorescent Ca²⁺ Indicators Excited with Visible Light—Section 19.3
• Indicators for Ca²⁺, Mg²⁺, Zn²⁺ and Other Metal Ions—Chapter 19

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

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