Thermo Fisher Scientific GAPDH Polyclonal Antibody
Rabbit polyclonal antibody against GAPDH for IHC and IHC-P applications. Reacts with human and mouse, predicted to cross-react with several species. Useful as a loading control in Western blot and research on glycolysis and neurodegenerative diseases.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 250 and 300 of human Glyceraldehyde-3-Phosphate Dehydrogenase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.10 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, predicted to react with Rat, Chicken, Turkey, Guinea pig, Pig, Monkey, Gorilla, Chimpanzee, Crab-eating macaque, Neobatrachia, and Sauropsida
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis. It exists as a tetramer of identical 37 kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate in the presence of NADPH.
Beyond glycolysis, GAPDH is ubiquitously expressed and participates in various cellular processes, including:
- DNA replication and repair
- Nuclear RNA export
- Membrane fusion and microtubule bundling
- Gene expression during apoptosis
- Cellular mechanisms in age-related neurodegenerative diseases
GAPDH also interacts with amyloid precursor protein (linked to Alzheimer’s disease) and Huntingtin (linked to Huntington’s disease). Due to its high expression in most tissues, GAPDH is widely used as a protein loading control in Western blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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