Thermo Fisher Scientific BaculoDirect C-Term Transfection Kit

Invitrogen™ BaculoDirect™ C-Term Transfection Kit

The BaculoDirect™ Baculovirus Expression System is a powerful and versatile eukaryotic system for high-level protein expression in insect cells. The combination of Gateway™ Technology with baculovirus expression makes the BaculoDirect System the fastest and easiest method for production of recombinant baculovirus.

How It Works

BaculoDirect™ Linear DNA (the baculovirus genome) is engineered to include attR sites for quick and efficient recombination with a Gateway entry clone.
The gene of interest is recombined from the entry clone into the BaculoDirect Linear DNA using a simple, one-hour LR reaction.
The resulting recombinant baculovirus carrying the gene of interest is then used to transfect insect cells.

This system eliminates the need for:

• Transforming bacteria and isolating a large bacmid
• Co-transfecting a transfer vector and linear baculovirus DNA

As a result, hands-on time is greatly reduced and purified baculovirus can be isolated in less than one week.

Gateway Linear DNA Features

• Strong polyhedrin promoter for high-level expression
• attR sites for efficient recombination with any attL-flanked Gateway entry vector
• TK gene for negative selection using ganciclovir
• C-terminal V5-His tag (C-Term Kit) or N-terminal V5-His tag (N-Term Kit) for detection with anti-V5 antibody and purification with nickel-chelating resin
• TEV protease cleavage site for removal of the V5-His tag following purification (N-Term Kit)
• LacZ gene to ensure a pure baculovirus stock is generated, which is replaced by your gene of interest after LR recombination

Additional materials required (available separately): Gateway entry clone.

A selection guide for choosing the most appropriate Gateway entry vector for your application can be found at:
www.thermofisher.com/Gateway

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