Thermo Fisher Scientific GAPDHS Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 179-408 of human GAPDHS (NP_0551791) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GAPDHS,uniProtId:O14556-1,ncbiNodeId:9606,antigenRange:179-408,antigenLength:408,antigenImageFileName:PA5-87928_GAPDHS_O14556-1_Rabbit.svg,antigenImageFileNamePDP:PA5-87928_GAPDHS_O14556-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

4.49 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804511

Product Specific Information

Immunogen sequence: ASDHISAGAQ RVVISAPSPD APMFVMGVNE NDYNPGSMNI VSNASCTTNC LAPLAKVIHE RFGIVEGLMT TVHSYTATQK TVDGPSRKAW RDGRGAHQNI IPASTGAAKA VTKVIPELKG KLTGMAFRVP TPDVSVVDLT CRLAQPAPYS AIKEAVKAAA KGPMAGILAY TEDEVVSTDF LGDTHSSIFD AKAGIALNDN FVKLISWYDN EYGYSHRVVD LLRYMFSRDK; Positive Samples: HT-1080, U-251MG, SW620, MCF-7, Mouse liver, Rat testis; Cellular Location: Cytoplasm

Target Information

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important house keeping functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimers disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntingtons disease. Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.