Thermo Fisher Scientific PKC epsilon Polyclonal Antibody, Biotin
PKC epsilon 단백질을 인식하는 Biotin 결합 토끼 다클론 항체. Western blot, ELISA, IP에 적합하며 인간 및 영장류 시료에 반응. 합성 펩타이드 면역원으로 제작되어 높은 특이성과 재현성을 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 350–400 of human Protein Kinase C epsilon (PRKCE, UniProt ID: Q02156-1) |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The PKC family of serine/threonine kinases, including PRKCE (PKC epsilon), is activated by intracellular signal transduction pathways. In humans, at least 12 PKC isoforms have been identified, differing in structure, tissue distribution, localization, and substrate specificity. Conventional PKC isoforms (alpha, beta I, beta II, gamma) are Ca²⁺- and phospholipid-dependent. PKC enzymes phosphorylate diverse protein targets, participate in multiple signaling pathways, and act as receptors for tumor-promoting phorbol esters. Each PKC isoform exhibits distinct cellular expression and function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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