Thermo Scientific™

Pierce™ ECL Western Blotting Substrate

Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that자세히 알아보기

Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Pierce ECL Plus Western Blotting Substrate characteristics include:
• Sensitivity: low picogram
• Stability: 1-hr working solution stability, 1-year kit stability at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 1 to 2 hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–10 μg/mL)
• Recommended secondary antibody concentration: 1:1,000–1:15,000 dilution (0.07–1.0 μg/mL)

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Pierce ECL Western Blotting Substrate provides reliability and performance equivalent to other standard ECL substrates for detection of HRP enzyme activity. It enables the detection of picogram amounts of antigen and allows for easy detection of HRP using photographic or other imaging methods.

Because the luminol and peroxide reagent formulations are identical to other commercially available substrate products, one can switch to Pierce ECL without needing to optimize probing conditions or incubation protocols. Pierce ECL Western Blotting Substrate provides performance identical to the original Amersham ECL Western Blotting Detection Reagent from GE Healthcare.

사양

설명Pierce ECL Western Blotting Substrate

제품 유형Substrate

검출 방법Chemiluminescence

형태Liquid

용도(애플리케이션)Western Blot

Membrane CompatibilityNitrocellulose, PVDF

제품라인Pierce™

수량500 mL

콘주게이트HRP

SubstrateHorseradish Peroxidase

권장 항체 농도2°: 1:1-15K (0.07-1μg/mL), 1°: 1:1K (0.2- 10μg/mL)

민감도Low Picogram

신호 지속 시간1 to 2 hr.

충분5000 sq. cm

키트 구성품Luminol/Enhancer, 250mL, Stable Peroxide Buffer, 250mL

Unit Size500 mL