Thermo Fisher Scientific LIN28A Monoclonal Antibody (GT717)
LIN28A 단백질을 검출하기 위한 Mouse monoclonal antibody (Clone GT717). Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합. Human 및 Mouse 반응성 확인. Recombinant LIN28 중심 영역을 면역원으로 사용. 연구용으로만 사용 가능.
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Thermo Fisher Scientific LIN28A Monoclonal Antibody (GT717)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Flow Cytometry (Flow) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT717 |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human LIN28 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787093 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (98%), Rat (98%), Cat (96%), Pig (96%), Chicken (82%), Sheep (97%), Bovine (96%).
- Positive Control: NT2D1, human ESC, LIN28 transfected HeLa lysates, mouse placenta.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Lin28 is a transcription factor first identified for its role in developmental timing in C. elegans. It plays a key role in stem cell generation and has potential applications in regenerative medicine. Artificial stem cells (induced pluripotent stem cells, iPS) can be created by expressing Lin28 along with transcription factors POU5F1, Sox2, and NANOG in mouse fibroblasts. Recent studies show iPS cells can be generated using plasmids expressing Lin28, Sox2, POU5F1, and c-Myc, eliminating viral introduction and improving safety for potential therapeutic use.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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