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Norgen Endotoxin Removal Kit (Maxi) - For DNA
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Norgen

Norgen Endotoxin Removal Kit (Maxi) - For DNA

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DNA 시료의 엔도톡신을 신속하게 제거하여 트랜스펙션 등 민감한 실험에 적합. 스핀 컬럼 방식으로 30분 내 처리 가능. 최대 1 mg DNA 처리 및 0.1 EU/µg 이하의 엔도톡신 수준 달성. 회수율 90% 이상으로 높은 효율 제공.

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Norgen Endotoxin Removal Kit (Maxi) - For DNA판매 단위 pk ·
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Norgen · Norgen Endotoxin Removal Kit (Maxi) - For DNA

Norgen Endotoxin Removal Kit (Maxi) - For DNA

SKU: 21900
For the rapid removal of endotoxins from previously purified DNA.

Features and Benefits

  • Reduce endotoxin levels to 0.1 EU/µg DNA or less
  • Available in 3 formats: Mini, Midi, and Maxi
  • Remove endotoxins from up to 1 mg of DNA with the Maxi format
  • Fast and easy processing using a rapid spin-column format

These kits are designed for rapid spin column removal of endotoxins from previously purified DNA. Norgen’s spin columns bind DNA while endotoxins, salts, and other contaminants are washed away. The kits reduce endotoxins to 0.1 EU/μg DNA or less, providing plasmid DNA ready for transfection or other endotoxin-sensitive applications. Typical DNA recovery is greater than 90% of the starting sample.

Kit Variants

Endotoxin Removal Kit (Mini)
Removes endotoxins from up to 25 µg of DNA. Procedure completed in ~20 minutes.

Endotoxin Removal Kit (Midi)
Removes endotoxins from up to 200 µg of DNA. Procedure completed in ~30 minutes.

Endotoxin Removal Kit (Maxi)
Removes endotoxins from up to 1 mg of DNA. Procedure completed in ~30 minutes.

About Endotoxins

Endotoxins (lipopolysaccharides) are components of Gram-negative bacterial cell membranes such as E. coli. They are released during plasmid purification and can reduce transfection efficiency in sensitive cell lines. Therefore, removing endotoxins is critical before downstream applications.

Supporting Data

Figure 1. Efficiently Remove Endotoxins
An input of 11 µg plasmid DNA (endotoxin >8 EU/µg) was cleaned using Norgen’s kit. Endotoxin levels were reduced to ≤0.1 EU/µg with high DNA recovery.

그래프 요약 (OCR 텍스트 반영):
| 구분 | Average EU/µg Plasmid (대략적 값) |
|------|----------------------------------|
| Before | 약 8.2 |
| After | 약 0.1 |

Figure 2. High DNA Recovery
Recovery of plasmid DNA was >90% after cleanup using the kit.

그래프 요약 (OCR 텍스트 반영):
| 항목 | % Recovery (대략적) |
|------|--------------------|
| Input DNA | 약 100% |
| Recovered DNA | 약 90% |

Kit Specifications

항목 사양
Maximum DNA Input 1 mg
Final Endotoxin Level ≤ 0.1 EU/µg DNA
Size of DNA Purified Up to 13,000 bp
Maximum DNA Volume Input 3 mL
Average Recovery >90%
Time to Complete 4 Purifications 30 minutes

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature.
This kit is stable for 2 years after shipment.

Kit Components

Component Cat. 22700 (25 preps) Cat. 52200 (10 preps) Cat. 21900 (4 preps)
Buffer SK 15 mL 60 mL 60 mL
Wash Solution H 18 mL 18 mL 18 mL
Elution Buffer I 6 mL 12 mL 12 mL
Endotoxin Removal Solution 1.5 mL 1.5 mL 1.5 mL
Precipitation Solution - 1.5 mL 1.5 mL
Spin Columns (assembled with Collection Tubes) - 10 4
Spin Columns 25 - -
Collection Tubes 25 - -
Elution Tubes (1.7 mL) 25 - -
Elution Tubes (15 mL) - 10 -
Elution Tubes (50 mL) - - 4
Product Insert 1 1 1

Documentation

Protocols

Safety Data Sheet

Flyer

FAQs

Why do I have poor DNA recovery?

Possible causes:

  • DNA did not bind properly to the column (check Buffer SK for precipitates)
  • Ethanol not properly added to Wash Solution H
  • Incomplete removal of Wash Solution H (ensure full spin time)
  • Incorrect Buffer SK volume (5:1 ratio to DNA volume)

Why is purified DNA not performing well?

  • DNA not washed with provided Wash Solution H
  • Incorrect or non-optimized elution buffer used
  • Elution buffer containing interfering components (e.g., salts, detergents)

Why are endotoxin levels slightly higher than 0.1 EU/µg?

  • Non-standard elution buffer used
  • Extremely high initial endotoxin levels (repeat purification if needed)

Citations

Title Citation Authors
Rational design and construction of multi-copy biomanufacturing islands in mammalian cells Nucleic Acids Research, 2022 Altamura R, Doshi J, Benenson Y
Precision multidimensional assay for high-throughput microRNA drug discovery Nature Communications, 2016 Benjamin Haefliger, Laura Prochazka, Bartolomeo Angelici, Yaakov Benenson
Transplantation of prokaryotic two-component signaling pathways into mammalian cells PNAS, 2014 J Hansen, E Mailand, KK Swaminathan, J Schreiber, B Angelici, Y Benenson

Genomic DNA Isolation Kit

  • Cat. 24700, 50 Preps
  • Includes Digestion Buffer A (15 mL), Wash Solution A (18 mL), Buffer SK (30 mL)
  • Store at room temperature
구성품 내용량 보관 조건 경고 표시
Digestion Buffer A 15 mL Room temperature -
Wash Solution A 18 mL Room temperature -
Buffer SK 30 mL Room temperature DANGER

Fungi/Yeast Genomic DNA Isolation Kit
Phage DNA Isolation Kit

제품 이미지

Figure 1. Efficiently Remove Endotoxins
Figure 2. High DNA Recovery
Genomic DNA Isolation Kit

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