Thermo Fisher Scientific ATG3 Monoclonal Antibody (OTI4F6), TrueMAB
ATG3 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. 인간 시료 반응성, 동결건조 형태로 제공되며, 연구용으로 자가포식 관련 단백질 분석에 적합.
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Thermo Fisher Scientific ATG3 Monoclonal Antibody (OTI4F6), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4F6 |
| Immunogen | Full-length human recombinant protein of human ATG3 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
ATG3 (APG3) is a conjugating enzyme essential for APG8 lipidation, a key step in autophagy initiation. Autophagy facilitates the degradation of cellular proteins via the autophagosomal-lysosomal pathway, crucial for normal growth control and cellular homeostasis.
Defects in autophagy may occur in tumor cells. It supports nutrient preservation under starvation and normal cytosolic turnover.
ATG3 acts as an E2-like enzyme catalyzing the formation of Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, critical for autophagosome formation.
This process is negatively regulated by TOR through phosphorylation of autophagy protein APG1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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