Thermo Fisher Scientific Phospho-53BP1 (Ser29) Polyclonal Antibody
Phospho-53BP1 (Ser29) 폴리클로날 항체로, 인간·마우스·비인간 영장류 시료에서 인산화된 53BP1 검출에 적합. WB, IHC, ICC/IF에 사용 가능하며, 고순도 정제 및 안정한 PBS/glycerol 저장용액 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human TP53BP1 (Accession Q12888), corresponding to amino acid residues around phosphorylated Ser29 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2903174 |
Product Specific Information
This antibody detects endogenous levels of 53BP1 only when phosphorylated at Ser29.
Target Information
53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to the presumptive sites of damage, specifically double-strand breaks. 53BP1 may have a role in checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and participates in DNA repair by maintaining genomic stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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