Thermo Fisher Scientific Vinculin Polyclonal Antibody
Thermo Fisher Scientific의 Vinculin Polyclonal Antibody는 인간, 마우스, 랫트에 반응하며 WB, ICC/IF, ELISA에 적합합니다. 세포 접착 및 세포골격 관련 연구에 활용되며, 고순도 친화 크로마토그래피 정제 항체로 안정적인 결과를 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 751–850 of human Vinculin (NP_054706.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.91 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805717 |
Product Specific Information
- Positive Samples: 293T, H460, HepG2, BxPC-3, Romas, U-87MG, Mouse lung, Mouse heart, Mouse brain
- Cellular Location: Cell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, adherens junction, cytoskeleton, focal adhesion
Immunogen sequence:
AGATSIARRA NRILLVAKRE VENSEDPKFR EAVKAASDEL SKTISPMVMD AKAVAGNISD PGLQKSFLDS GYRILGAVAK VREAFQPQEP DFPPPPPDLE
Target Information
Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. Vinculin interacts with talin and alpha-actinin, contributing to the transmembrane linkage between the extracellular matrix and the cytoplasmic microfilament system. Mutations in the VCL gene may lead to cardiomyopathy dilated 1W or familial hypertrophic 15.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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