Thermo Fisher Scientific CaV1.1 Polyclonal Antibody
Rabbit polyclonal antibody against human CaV1.1 (CACNA1S). Validated for IHC (paraffin) with 1:200–1:500 dilution. Antigen affinity purified, supplied in PBS with 40% glycerol. Suitable for research on calcium channel function and excitation–contractio...
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Applications
Tested Application and Dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human CACNA1S. Recombinant protein control fragment (Product # RP-99773) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2791464 |
Product Specific Information
Immunogen sequence:
EEEKSTMAKK LEQKPKGEGI PTTAKLKIDE FESNVNEVKD PYPSADFPGD DEEDEPEIPL SPRPRPLAEL
Target Information
Voltage-sensitive calcium channels mediate the entry of calcium into many types of excitable cells and thus play a key role in neurotransmitter release and excitation–contraction (E–C) coupling.
The 1,4-dihydropyridines (DHPs) are synthetic organic compounds used to identify L-type calcium channels found in various muscle, neuronal, and neuroendocrine cells.
The DHP receptor, part of the L-type calcium channel complex, acts as the voltage sensor in E–C coupling. The purified DHP receptor isolated from triads is composed of at least four subunits:
- Alpha-1 subunit: Contains the binding site for DHPs; shows high sequence homology to voltage-gated sodium channels.
- Alpha-2 subunit: Large glycoprotein associated with the DHP receptor; abundant in skeletal and cardiac muscle, brain, and other excitable tissues.
- Beta and gamma subunits: Co-purify with the DHP receptor and contribute to its function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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