Thermo Fisher Scientific WEE2 Polyclonal Antibody
Human WEE2 단백질을 인식하는 Rabbit Polyclonal Antibody로, IHC(P) 및 ICC/IF에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS(40% 글리세롤) 버퍼에 보관됩니다. 단기 4°C, 장기 -20°C에서 보관 권장. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human WEE2. Recombinant protein control fragment (Product # RP-95085) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2649640 |
Product Specific Information
Immunogen sequence:
TKSGNHFEEP KLKDILLQIS LGLNYIHNSS MVHLDIKPSN IFICHKMQSE SSGVIEEVEN EADWFLSANV MYKIGDLGHA TSINKPKVEE GD
Highest antigen sequence identity to orthologs:
- Mouse: 83%
- Rat: 82%
Target Information
Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1/CDC2, acting as a key regulator of meiosis during both prophase I and metaphase II (PubMed:29606300).
Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage by phosphorylating CDK1 at Tyr-15, inhibiting CDK1 activity and preventing meiotic reentry.
Also required for metaphase II exit during egg activation by phosphorylating CDK1 at Tyr-15, ensuring exit from meiosis and promoting pronuclear formation.
[Source: UniProt]
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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