Thermo Scientific™

GeneJET Plasmid Midiprep Kit

Thermo Scientific GeneJET Plasmid Midiprep Kit, sufficient for 25 preps, is designed for large scale isolation of high quality plasmid자세히 알아보기

Thermo Scientific GeneJET Plasmid Midiprep Kit, sufficient for 25 preps, is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 μg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights
• Efficient—high yields up to 200 μg of high quality plasmid DNA from 50 mL of bacterial culture
• High concentration of DNA purified— >400 ng/μL
• Convenient—spin column format, centrifuge or vacuum protocols
• Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications
Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
• FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
• In vitro transcription
• Transfection of robust cell lines

사양

농도400 ng/μL

컬럼 유형Spin Column

형식Spin Column

Isolation TechnologySilica Column

스케일Midi

샘플 종류Bacteria

용도(애플리케이션)Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR

고처리량 호환성Not High-throughput Compatible (Manual)

프렙 스케일100-200 μg (Medium-Scale) Plasmid DNA

제품라인GeneJET

제품 유형Plasmid MidiPrep Kit

수량25 Preps

타겟Plasmid DNA

테스트 시간60 min.

Unit SizeEach