Thermo Fisher Scientific MCM3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000-1:25,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000-1:5,000

-

Immunoprecipitation (IP)

2-10 µg/mg lysate

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residues 500 and 550 of human Minichromosome Maintenance 3. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MCM3,uniProtId:P25205-1,ncbiNodeId:9606,antigenRange:500-550,antigenLength:808,antigenImageFileName:A300-192A_MCM3_P25205-1_Rabbit.svg,antigenImageFileNamePDP:A300-192A_MCM3_P25205-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

phosphate/tris citrate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Based on 100% sequence identity, this antibody is predicted to react with Orangutan

Target Information

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.