Invitrogen™

NuPAGE™ Large Protein Blotting Kit

The HiMark™ Pre-Stained and Unstained High Molecular Weight Protein Standards are specifically designed for large protein analysis on NuPAGE™ Novex자세히 알아보기

The HiMark™ Pre-Stained and Unstained High Molecular Weight Protein Standards are specifically designed for large protein analysis on NuPAGE™ Novex Tris-Acetate Gels under denaturing conditions. Both standards offer a time-saving load-and-go format.

The HiMark™ Pre-stained Standard includes one pink colored orientation band and eight blue bands ranging in apparent molecular weight of 31 - 460 kDa* in NuPAGE™ Tris-Acetate Gel/SDS buffer system (Figure 1). It`s suitable for monitoring electrophoresis, western transfer, and approximating molecular weight of unknown big proteins.

The HiMark™ Unstained Standard, along with the NuPAGE™ Tris-Acetate Gel/SDS buffer system, offers the highest accuracy for molecular weight estimation of large proteins. It consists of nine proteins, ranging in size from 40 to 500 kDa in NuPAGE™ Novex 3-8% and 7% Tris-Acetate Gel/SDS buffer systems (Figure 2). Visualize with Coomassie™ stain, silver, and fluorescent protein stains post-electrophoresis. A downloadable molecular weight calculator is designed for easy and accurate MW estimation of large proteins using the HiMark™ Unstained Standard on a NuPAGE™ Tris-Acetate Gel.**

Several NuPAGE™ Large Protein Starter Kits are available to provide you with all necessary reagents and optimized protocols for highresolution large protein separation and analysis (Table 1). Each kit includes two boxes of NuPAGE™ 3-8% Tris-Acetate Gels, Tris-Acetate SDS Buffer Kit, a staining reagent or blotting membranes, and a vial of the appropriate HiMark™ Standard.

사양

용도 (장비)XCell SureLock Mini-Cell

젤 비율3 to 8%

젤 크기Mini

젤 유형Tris-Acetate

제품 유형Protein Blotting Kit

샘플 로딩 부피25 μL

분리 범위40 to 500 kDa

염색 유형Unstained

웰10-well

제품라인NuPAGE™

수량20 gels kit

두께1.0 mm

웰 디자인1D Well

Unit SizeEach